Ikhithi isetyenziselwa ukukhangela umgangatho we-in vitro wokukhangela i-ORF1ab/N/S yofuzo yenoveli coronavirus 2019-nCoV kwimizekelo yokuphefumla equka i-oropharyngeal swabs, i-nasopharyngeal swab, isikhohlela kunye nokwenza uguqulelo lokuchwetheza kwi-HV69- 70 del ngaxeshanye.Njengoko kuxeliwe kwi-database ye-GISAID, ngaphezu kwe-95% ye-Omicron ehlukeneyo yolandelelwano oluxeliweyo lubandakanya ukucinywa kwi-HV69-70, enokubangela ukusilela kwe-S gene target (SGTF) kwiimvavanyo ze-PCR.I-SGTF inokusetyenziswa njengesiphawuli sommeli ukukhusela i-Omicron.
Iiseti zePrimer kunye ne-FAM ebhalwe probe ziyilelwe ukuchongwa ngokuthe ngqo kohlobo lwelebhu ye-ORF ka-2019-nCoV, i-VIC ebhalwe i-N gene ka-2019-nCoV, i-ROX ebhalwe probe ye-S gene HV69-70 del mutation ka-2019-nCoV.I-gene ye-RNase ye-P yomntu ekhutshwe ngaxeshanye kunye nesampuli yovavanyo inika ulawulo lwangaphakathi lokuqinisekisa inkqubo ye-nucleic extraction kunye nokuthembeka kwe-reagent.Uphando olujolise kuhlobo lwe-RNase P yomntu lubhalwe nge-CY5.
Inkqubo yePCR yexesha langempela: Aehealth Diagenex AL, ABI 7500, ViiATM 7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-Gene TM6000/0.I-Bio-Rad CEX96 Touch TM SLAN-96S.SLAN-96P, MA 6000;