Le khithi isetyenziselwa ukufumanisa i-in vitro qualitative nucleic acid ye-Monkeypox Virus kunye ne-central/west African clade kwi-Human serum, iisampuli ze-lesion exudate kunye ne-scab specimens.Iiseti zePrimer kunye ne-FAM ezibhalwe probe ziyilelwe ukuchongwa okuthe ngqo kweMonkeypox Virus, ROX ebhalwe probe kumbindi weAfrika, CY5 ebhalwe probe yeWest Africa clade.I-gene ye-RNase ye-P yomntu ekhutshwe ngaxeshanye kunye nesampuli yovavanyo inika ulawulo lwangaphakathi lokuqinisekisa inkqubo ye-nucleic extraction kunye nokuthembeka kwe-reagent.Uphando olujolise kwijini ye-RNase P yomntu ibhalwe nge-VIC.
| Amacandelo | 48 Iimvavanyo/ikhithi | 96 Iimvavanyo/ikhithi |
| PCR reaction buffer | 672 μL × 1 ityhubhu | 672 μL × 2 ityhubhu |
| PCR enzyme mix | 50 μL × 1 ityhubhu | 100 μL × 1 ityhubhu |
| Ulawulo oluhle | 100 μL × 1 ityhubhu | 200 μL × 1 ityhubhu |
| Ulawulo olubi | 100 μL × 1 ityhubhu | 200 μL × 1 ityhubhu |
1. Uvakalelo: Iikopi ezingama-200/mL.
2. Ukuchaneka okucacileyo: Akukho kusabela okunqamlezileyo nge-Enterovirus(EV), intsholongwane yemasisi(MV), Rubella virus(RV), Varicella-zoster virus(VZV), Dengue virus(DenV), Human Parvovirus B19(HPVB19), Epstein-barr virus (EBv), intsholongwane ye-herpes yomntu 6(HHV-6)
3. Ukuchaneka: I-CV ≤ 5%.
Inkqubo yePCR yexesha langempela:Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-Gene TM 6000/Q, Bio-Rad CFX96 TouchTM/iQTM ，Hongshi SLAN -96S/96P,AGS8830, AGS4800