Le khithi isetyenziselwa ukufumanisa i-in vitro qualitative nucleic acid ye-2019-Novel Coronavirus (2019-nCoV), uMkhuhlane A kunye nomkhuhlane B ngaxeshanye kwisampulu zokuphefumla kubandakanya i-oropharyngeal swabs kunye nesikhohlela.Iiseti zePrimer kunye ne-FAM ebhalwe probes ziyilelwe ukuchongwa okuthe ngqo kuzo zombini iORFlab kunye ne-N genes ka-2019-nCoV, i-VIC ebhalwe probe ye-RNase P gene njengolawulo lwangaphakathi, i-ROX ebhalwe i-probe ye-M gene ye-Influenza A, i-CY5 ebhalwe probe ye-NS gene. kuMkhuhlane B.
| Amacandelo | Iimvavanyo ezingama-50/ikhithi |
| RT-PCR reaction buffer | 915 μL × 1 ityhubhu |
| Umxube we-enzyme ye-RT-PCR | 75 μL × 1 ityhubhu |
| Ulawulo oluhle | 100 μL × 1 ityhubhu |
| Ulawulo olubi | 100 μL × 1 ityhubhu |
Uvakalelo:200 iikopi/mL.
Ukucaciswa: Akukho mpendulo inqamlezileyo kunye ne-SARS-CoV, i-MERS-CoV, i-CoV-HKU1, i-CoV-OC43, i-CoV-229E, i-CoV-NL63, i-Parainfluenza Virus, i-Human Adenovirus, i-Human Rhinovirus, i-Respiratory Syncytial Virus (A), i-Respiratory Syncytial Intsholongwane (B), Enterovirus A, Staphylococcus Aureus, Streptococcus Pneumonia, Klebsiella Pneumonia.
Ukuchaneka: CV ≤ 5%.
Inkqubo yePCR yexesha lokwenyani:I-Aehealth Diagenex AL,Molarray MA-6000, ABI 7500, ViiATM7, QuantStudio 7 flex, Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/Q, Bio-Rad CFX96 TouchTM/iQTM5，SLAN-96S/96P