| 64T | 96T | ||
| Icandelo | Umthamo | Icandelo | Umthamo |
| Ipleyiti ye-reagent | 4 | ULysis buffer B | 2 |
| ULysis buffer B | 1 | Isitya seLysis | 1 |
| Umkhono weplastiki | 8 | Hlamba ipleyiti enye | 1 |
| Incwadi yemigaqo | 1 | Hlamba iipleyiti ezi-2 | 1 |
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| Ipleyiti yeElution | 1 |
|
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| Umkhono weplastiki | 1 |
|
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| Incwadi yemigaqo | 1 |
96-kahle Round Hole Plate Ukulungiselela
Izixhobo ze-64T kwipleyiti yomthombo ohambelanayo ngolu hlobo lulandelayo:
| Indawo entle | 10r7 | 2or8 | 3 okanye 9 | 4or10 | 5orll | 6orl2 |
| Ikhithi Icandelo | ULysis Isithinteli 600μL | Hlamba Isithinteli1 500μL | Hlamba Buffer2 500μL | Akunanto | Imagnethi Amaso 310μL | Elute Isithinteli l00μL |
Fokanye 64T ikhithi:
Susa ngononophelo ifilimu yokutywina ubushushu kwipleyiti yereagent, uze wongeze i-200μL yesampuli kunye ne-20μL ye-lysis buffer B kwikholamu ye-1/7 yepleyiti ye-reagent.
Ngekhithi ye-96T:
Susa ngononophelo ifilimu yokutywina ubushushu kwipleyiti ye-reagent, kwaye wongeze i-200μL yesampuli kunye ne-20μL ye-lysis buffer B kwipleyiti ye-lysis.
Faka ipleyiti ye-reagent kunye nomkhono weplastiki kwindawo echongiweyo yesixhobo ngokulandelelana, kwaye emva koko ucofe ukuqhuba "i-DNA / RNA" inkqubo yokukhutshwa kwi-nucleic acid extractor.
Ekupheleni kwenkqubo, khupha umkhono weplastiki kwaye uwulahle.
Thatha ipleyiti elution, kwaye eluent itsalwa kwaye igcinwe kwityhubhu entsha ye-centrifuge kwimifuniselo esezantsi.Ukuba umfuniselo osezantsi awunakwenziwa ngexesha, isampuli yeDNA inokugcinwa ku -20℃ kwaye isampulu yeRNA inokugcinwa ku -80℃.