I-Spike glycoprotein receptor-binding domain (RBD) ye-SARS-CoV-2 ilamla ukubophelela kwesuntswana lentsholongwane kwi-angiotensin-converting enzyme 2 (ACE2) receptor kumphezulu weeseli zomntu.Ke ngoko, ukusebenzisana kwe-Spike-ACE2 yeyona nto ibalulekileyo yokumisela infectivity yentsholongwane.Iqela elitsha le-phylogenetic ye-SARS-CoV-2 (umnombo B.1.1.7), ebonisa ukutshintshwa kwe-amino acid kwi-Spike RBD (N501Y mutation).
Ngokophando olutsha oluvela kwiSebe lezeMpilo lase-UK, ngokusekwe kwidatha yolandelelwano lwe-genome epheleleyo, i-epidemiology kunye nemodeli, ukosuleleka kohlobo olwahlukileyo lwe-SARS-CoV-2 luphantse lube ngama-40-70% ngaphezulu kunolunye usosayensi ofunyenweyo wohlengahlengiso. .Ukusabela ngokukhawuleza kunye nokufunyanwa yenye yezinto ezibalulekileyo zokuthintela kunye nomlinganiselo wokulawula.
Ikhithi isetyenziselwa i-in vitro qualitative ukukhangela i-ORF1ab/N/S yofuzo yenoveli coronavirus 2019-nCoV kwisampulu zokuphefumla, kunye nokwenza uguqulelo lokuchwetheza ku-N501Y ngaxeshanye.
Ikhithi yamkela indlela ye-ARMS, ithatha i-S gene yenoveli coronavirus (2019-nCoV) njengendawo ekujoliswe kuyo yokuchwetheza ngotshintsho, iyila iiprimers ezithile kunye neprobes ye-N501Y.
| Iimveliso | Iinkcukacha | Ubushushu bokugcina | Inkcazo |
| Ixesha lokwenyani leKhithi yePCR yeNoveliICoronavirus 2019-nCoV kunyeInoveli yeCoronavirus ka-2019-nCoVUkuguqulwa kweGene (N501Y) | 48T | -20℃ | Abahlalutyi: Udidi lwasendle lwe-N501Y,Uhlobo oluguqukayo lwe-N501Y, ORF1ab,I-N gene kunye nolawulo lwangaphakathi |
Ukuhambelana kwetemplate
- I-Oropharyngeal Swabs
- I-Nasopharyngeal Swab
- Bronchoalveolar Lavage Fluid
- Isikhohlela
Izixhobo zeKit
- RT-PCR reaction buffer
- Umxube we-enzyme ye-RT-PCR
- Ulawulo olulungileyo lwendalo yasendle
- Ulawulo olulungileyo olutshintshiweyo
- Ulawulo olubi
N501Y Uhlobo loGuquko
