Inoveli coronaviruses zezohlobo B.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla.Ngokuqhelekileyo abantu basengozini.Okwangoku, izigulana ezosulelwe yinoveli coronavirus ngoyena mthombo wosulelo;abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela yi-1 ukuya kwiintsuku ze-14, ubukhulu becala iintsuku ezi-3 ukuya kwezi-7.Iimpawu eziphambili ziquka umkhuhlane, ukukhathala kunye nokukhwehlela okomileyo.Ukuxinana kweempumlo, iimpumlo, umqala obuhlungu, i-myalgia kunye ne-diarrhea zifumaneka kwiimeko ezimbalwa.
Le khithi isetyenziselwa ukufumanisa umgangatho we-in vitro wenoveli coronavirus 2019-nCov kwisampulu zokuphefumla ezibandakanya i-oropharyngeal swab, isikhohlela, i-bronchoalveolar lavage fluid kunye ne-nasopharyngeal swab.Iiseti zePrimer kunye ne-FAM ebhalwe probe ziyilelwe ukuchongwa kofuzo oluthile lwe-ORLab ye-2019-nCov.I-VIC ebhalwe ngophendlo lwe-N gene ka-2019-nCov.I-gene ye-RNase ye-P yomntu ekhutshwe ngaxeshanye kunye nesampuli yovavanyo inika ulawulo lwangaphakathi lokuqinisekisa inkqubo ye-nucleic extraction kunye nokuthembeka kwe-reagent.Uphando olujolise kuhlobo lwe-RNase P yomntu lubhalwe nge-CY5.
| Amacandelo | Iimvavanyo ezingama-50/ikhithi |
| RT-PCR Reaction Mix Reagent | 240μL × 1 ityhubhu |
| I-Enzyme Mix Reagent | 72μL × 1 ityhubhu |
| I-2019-nCoV primer probe | 48μL × 1 ityhubhu |
| Ulawulo Olulungileyo | 200μL × 1 ityhubhu |
| Ulawulo olubi | 200μL × 1 ityhubhu |
Uvakalelo: 200 iikopi/mL.
Ukucaciswa: Akukho mpendulo enqamlezileyo nge-SARS-CoV, MERS-CoV, CoV-HKU1, CoV-OC43, CoV-229E, CoV-NL63 kunye ne-HIN1, H3N2, H5N1, H7N9, Influenza B, Parainfluenza Virus (123), Rhinovirus (A) ,B,C), Adenovirus (1,2,3,4,5,7,55), Human interstitial pneumovirus, Human metapneumovirus, EBv, Intsholongwane yemasisi, Human cytomegalic virus, Rota virus, Norovirus, Mumps virus, Varicella Zoster Virus , Mycoplasma pneumonia, Chlamydia pneumonia, Legionella, Bordetella pertussis, Haemophilus influenza, Staplhylococcus Aureus, Streptococcus Pneumonia, Streptococcus pyogenes, Klebsiella inyumoniya, i-Tuberculous bacillus, Aspergillus fumibicantaccuses, Candidas, Capital, Capital, Capital, Capital, Caladida
Ukuchaneka: I-CV ≤5%.
Inkqubo yePCR yexesha lokwenyani: Aehealth Diagenex AL, ABI 7500, ViiATM7, Quant Studio 7 flex.Roche Lightcycler 480, Agilent Mx3000P/3005P, Rotor-GeneTM6000/0.Bio-Rad CEX96 TouchTMI-SLAN-96S.SLAN-96P