UVavanyo oluKhawulezayo lwe-COVID-19 ye-Antigen yi-colloidal yegolide ye-immunochromatography eyenzelwe ukufunyaniswa okusemgangathweni kwee-antigens ze-nucleocapsid ezivela kwi-COVID-19 kwi-swabs yeempumlo zabantu, i-swabs yomqala okanye amathe kubantu abarhanelwa ukuba bane-COVID-19 ngumboneleli wabo wezempilo.Inoveli coronaviruses yeyodidi lwe-β.I-COVID-19 sisifo esosulelayo esinamandla sokuphefumla.Ngokuqhelekileyo abantu basengozini.Okwangoku, izigulana ezosulelwe yinoveli coronavirus ngoyena mthombo wosulelo;abantu abosulelekileyo abangenazimpawu nabo banokuba ngumthombo wosulelo.Ngokusekelwe kuphando lwangoku lwe-epidemiological, ixesha lokufukamela yi-1 ukuya kwiintsuku ze-14, ubukhulu becala iintsuku ezi-3 ukuya kwezi-7.Iimpawu eziphambili ziquka umkhuhlane, ukukhathala kunye nokukhwehlela okomileyo.Ukuxinana kweempumlo, impumlo evuzayo, umqala obuhlungu, i-myalgia kunye ne-diarrhea zifumaneka kwiimeko ezimbalwa.Iziphumo zezochongo lwe-COVID-19 nucleocapsid antigen.I-antigen ibonakala ngokubanzi kwiisampuli eziphezulu zokuphefumla okanye iisampulu ezisezantsi zokuphefumla ngexesha lesigaba esinzima sosulelo.Iziphumo ezilungileyo zibonisa ubukho be-antigens yentsholongwane, kodwa ukulungelelaniswa kweklinikhi kunye nembali yesigulane kunye nolunye ulwazi lokuxilonga luyimfuneko ukufumanisa imeko yokusuleleka.Iziphumo ezilungileyo aziyikhuseli ngaphandle usulelo lwebhaktheriya okanye ukosuleleka kunye nezinye iintsholongwane.I-antigen efunyenweyo isenokungabi ngunobangela oqinisekileyo wesifo.Iziphumo ezibi azilukhupheli ngaphandle usulelo lwe-COVID-19 kwaye akufunekanga isetyenziswe njengesona siseko sonyango okanye izigqibo zolawulo lwesigulane, kubandakanywa nezigqibo zolawulo losulelo.Iziphumo ezibi kufuneka ziqwalaselwe kumxholo wokuvezwa kwesigulana mva nje, imbali kunye nobukho beempawu zeklinikhi kunye neempawu ezihambelana ne-COVID-19 kwaye zingqinwe ngovavanyo lwemolekyuli, ukuba kuyimfuneko kulawulo lwesigulana.
Le reagent isekwe colloidal igolide immunochromatography assay.Ngexesha lovavanyo, ii-extracts ze-specimen zisetyenziswa kwiiKhadi zoVavanyo.Ukuba kukho i-antigen ye-COVID-19 kwisicatshulwa, i-antigen iya kuzibophelela kwi-COVID-19 ye-monoclonal antibody.Ngexesha lokuhamba kwe-lateral, i-complex iya kuhamba kunye ne-nitrocellulose membrane ukuya ekupheleni kwephepha elithathayo.Xa upasa umgca wovavanyo (umgca T, oqatywe nenye i-COVID-19 monoclonal antibody) ikhompleksi ibanjwa li-antibody ye-COVID-19 kumgca wovavanyo ibonisa umgca obomvu;xa udlula umgca C, i-colloidal gold-labeled ibhokhwe echasene nomvundla i-IgG ibanjwe ngumgca wokulawula (umgca C, uhlanganiswe nomvundla we-IgG) ubonisa umgca obomvu.
La macandelo alandelayo abandakanyiwe kwikhithi yovavanyo ye-Rapid COVID-19 Antigen.
Izixhobo ezibonelelweyo:
| Uhlobo lweSampuli | Izinto eziphathekayo |
|
Amathe (kuphela) |
|
Izinto ezifunekayo kodwa azibonelelwanga:
1. Isibali-xesha
2. I-tube rack yeesampulu
1. Gcina imveliso kwi-2-30 ℃, ubomi beshelufu ziinyanga ezingama-24.
2. Ikhasethi yovavanyo kufuneka isetyenziswe kanye emva kokuvula isingxobo.
3. Ii-reagents kunye nezixhobo mazibe kwiqondo lobushushu begumbi (15-30℃) xa zisetyenziselwa uvavanyo.
Ukuqokelelwa koMfanekiso woMqala woMqala:
Vumela intloko yesigulana ithobe kancinci, uvule umlomo, kwaye wenze izandi "ah", eveza i-pharyngeal tonsils kumacala omabini.Bamba i-swab kwaye usule iitoni ze-pharyngeal kumacala omabini esigulane kunye namandla aphakathi emva naphambili ubuncinane be-3 ixesha.
Ingqokelela yoMfanekiso wamathe nguSwab:
Ukuqokelelwa koMfanekiso wamathe ngesiXhobo sokuQokelelwa kwamathe:
Umzekelo wezoThutho noGcino:
Iisampulu kufuneka zivavanywe ngokukhawuleza emva kokuqokelela.Iiswabs okanye umfuziselo wamathe ungagcinwa kwi-Extraction Solution ukuya kutsho kwiiyure ezingama-24 kwiqondo lobushushu begumbi okanye kwi-2° ukuya kwi-8°C.Musa ukuba ngumkhenkce.
1. Uvavanyo kufuneka luqhutywe kwindawo yokushisa (15-30 ° C).
2. Yongeza imizekelo.
Umzekeliso wamathe (usuka kwisixhobo sokuqokelelwa kwamathe):
Okuhle
Kukho umbala kumgca C, kwaye umgca onemibala uvele umgca T okhaphukhaphu kunomgca C, okanye phaya
Ibi
Kukho umbala kumgca C, kwaye umgca onemibala uvele umgca T omnyama okanye olingana no
Ayisebenzi
Akukho mibala kumgca C, njengoko kubonisiwe kule mifanekiso ilandelayo.Uvavanyo alusebenzi okanye luyimpazamo
Engalunganga(-): Iziphumo ezingalunganga ziyacingela.Iziphumo zovavanyo ezibi aziluthinteli usulelo kwaye akufuneki zisetyenziswe njengekuphela kwesiseko sonyango okanye ezinye izigqibo zolawulo lwesigulana, kubandakanywa izigqibo zolawulo losulelo, ngakumbi xa kukho iimpawu zekliniki kunye neempawu ezihambelana ne-COVID-19, okanye kwabo bebekhe bagula. ukudibana nentsholongwane.Kucetyiswa ukuba ezi ziphumo ziya kuqinisekiswa yindlela yokuvavanya i-molecular, ukuba kuyimfuneko, kuLawulo lokulawula isigulane.
I-Positive(+): Ilungile kubukho be-antigen ye-SARS-CoV-2.Iziphumo ezilungileyo zibonisa ubukho be-antigens yentsholongwane, kodwa ukulungelelaniswa kwekliniki kunye nembali yesigulane kunye nolunye ulwazi lokuxilonga luyimfuneko ukugqiba isimo sosulelo.Iziphumo ezintle azilukhuhli usulelo lwebhaktiriya okanye ukudibana nezinye iintsholongwane.I-antigen efunyenweyo isenokungabi ngunobangela oqinisekileyo wesifo.
1.Ukusebenza kweKlinikhi kwavavanywa ngeesampulu ezikhenkcezisiweyo, kwaye ukusebenza kovavanyo kunokwahluka ngeesampulu ezintsha.
2.Abasebenzisi kufuneka bavavanye iisampulu ngokukhawuleza emva kokuqokelelwa kwesampuli.
3. Iziphumo zovavanyo oluqinisekileyo azikuthinteli ukosuleleka kunye nezinye iintsholongwane.
4. Iziphumo zovavanyo lwe-antigen ye-COVID-19 kufuneka zidityaniswe nembali yezonyango, idatha ye-epidemiological, kunye nenye idatha ekhoyo kugqirha ovavanya isigulana.
I-5.Isiphumo sovavanyo olungeyonyani sinokuthi senzeke ukuba inqanaba le-antigen yentsholongwane kwisampuli lingaphantsi komda wokubona uvavanyo okanye ukuba isampuli iqokelelwe okanye ihanjiswe ngokungafanelekanga;ke ngoko, iziphumo zovavanyo ezingalunganga azikususi ukuba nokwenzeka kosulelo lwe-COVID-19.
6.Isixa se-antigen kwisampulu sinokuhla njengoko ixesha lokugula landa.Iisampulu eziqokelelwe emva komhla we-5 wokugula kunokwenzeka ukuba zingabikho xa kuthelekiswa novavanyo lwe-RT-PCR.
7.Ukungaphumeleli ukulandela inkqubo yovavanyo kunokuchaphazela kakubi ukusebenza kovavanyo kunye/okanye kungabi nakusebenza iziphumo zovavanyo.
8. Imixholo yale khithi iza kusetyenziselwa ukuchongwa okusemgangathweni kwee-antigens ze-COVID-19 kwisampulu zamathe kuphela.
9.I-reagent inokubona zombini i-antigen ye-COVID-19 esebenzayo nengasebenziyo.Ukubona ukusebenza kuxhomekeke kumthwalo we-antigen kwaye ayinakunxulumana nezinye iindlela zokuxilonga ezenziwa kumzekelo omnye.
10. Iziphumo zovavanyo olubi azenzelwanga ukulawula ezinye izifo ezingezizo i-COVID-19 yintsholongwane okanye yintsholongwane.
11. Amaxabiso aqikelelwayo amahle nabi axhomekeke kakhulu kumazinga okuxhaphaka.Iziphumo zovavanyo oluqinisekileyo zilindeleke ukuba zibonise iziphumo ezingeyonyani ngexesha lokwenzeka kancinci/kungabikho kwe-COVID-19 xa ukuxhaphaka kwezifo kusezantsi.Iziphumo zovavanyo ezingezizo ezingezizo zinokwenzeka xa ukuxhaphaka kwesifo esibangelwa yi-COVID-19 kuphezulu.
12. Esi sixhobo sivavanyelwe ukusetyenziswa nomfanekiso womntu kuphela.
13. Izilwa-buhlungu ze-Monoclonal zinokusilela ukufumanisa, okanye ukufumanisa ngovakalelo oluncinane, iintsholongwane ze-COVID-19 eziye zehla utshintsho olungephi lwe-amino acid kummandla we-epitope ekujoliswe kuwo.
14. Ukusebenza kolu vavanyo akuzange kuhlolwe ukuba kusetyenziswe izigulane ngaphandle kweempawu kunye neempawu zokusuleleka kokuphefumula kunye nokusebenza kunokwahluka kubantu abangenayo i-asymptomatic.
15. Ikhithi yaqinisekiswa ngeeswabs ezahlukeneyo.Ukusetyenziswa kwezinye iiswabs kunokukhokelela kwiziphumo ezingezizo ezingezizo.
16. Abasebenzisi kufuneka bavavanye iisampulu ngokukhawuleza emva kokuqokelelwa kwesampuli.
17. Ukuba semthethweni koVavanyo lwe-Antigen oluKhawulezayo lwe-COVID-19 alukangqinwa ukuze kuchongwe/ukuqinisekiswa kokwahlukaniswa kwezicubu zomzimba kwaye akufuneki kusetyenziswe kwesi sikhundla.