I-SARS-CoV-2 (COVID19) iyagquba kwihlabathi jikelele, kwaye ugonyo luvunywa njengeyona ndlela inoqoqosho nesebenzayo yokulawula ubhubhane wentsholongwane.Uvandlakanyo lwesitofu sogonyo lwesintu ikakhulu lusebenzisa iindlela zokubhaqa ii-antibody ukuze zivavanye ukusebenza kogonyo ngokusebenzisa imifuniselo yokungathathi hlangothi;
Iindlela zesiNtu zidla ixesha kwaye zisebenza kancinci, zihlala zithatha iintsuku ezi-2 ukuya kwezi-4 ukugqiba uphononongo, kwaye ngenxa yokuba uninzi lwazo zisebenzisa iintsholongwane eziphilayo, Kufuneka kuqhutywe kwinqanaba le-biosafety 3 okanye ngaphezulu kwilabhoratri, elixesha- idla kwaye ibulaleka, kwaye izisa inkxamleko enkulu kuvavanyo lokwandiswa nokuhlanganiswa.Ke ngoko, kukho imfuno engxamisekileyo yendlela elula nekhawulezayo eyenye ekulungeleyo ukuphononongwa kwezilwa-buhlungu ezikhuselayo kuluntu olukhulu.
I-Aehealth COVID19 I-Neutralization Antibody Quantitative Test Kit isetyenziselwa ukufunyaniswa ngokobungakanani be-COVID19 antibodies kwiserum yomntu, iplasma okanye igazi elipheleleyo.Ingasetyenziselwa ukufunyanwa ngokukhawuleza, ubuninzi kunye nobuthathaka kakhulu kwi-vitro.isetyenziswe eklinikhi kuvavanyo oluncedisayo lwesiphumo sesitofu esitsha se-coronavirus kunye novavanyo lwe-anti-anti-anti-anti-un-uncleing kwizigulana ezibuyiselweyo emva kosulelo.
Ii-antibodies ezingathathi hlangothi kulumisa ngokufanelekileyo usulelo ngokuthintela unxibelelwano phakathi kwentsholongwane ye-COVID19 kunye neeseli ezibambayo.Uninzi lwe-antibodies engathathi hlangothi luphendula kwi-domain binding domain (RBD) yeprotein ye-spike, ebophelela ngokuthe ngqo kwi-cell surface receptor ACE2.Amajoni omzimba-kwi-intanethi okwangoku abonelela ngezilwa-buhlungu ezibini zokungathathi hlangothi ezisekwe kwi-Clone CR3022.Ngelixa uninzi lwe-S-protein ye-RBD ebopha izilwa-buhlungu zikhuphisana ngokubophelela i-antigen nge-ACE2, i-CR3022 epitope ayidibani nesiza sokubopha i-ACE2.
Ngoko ke ayikuthinteli ukubophelela kwezilwa-buhlungu ezingathathi hlangothi.Ngelixa i-CR3022 ngokwayo ibonakalisa kuphela isiphumo esibuthathaka sokungathathi hlangothi, ibonakalisiwe isebenzisana nezinye ii-S-protein RBD ezibophelela izilwa-buhlungu zokulwa i-COVID19.
Ukusebenza lula
- Akukho mfuneko yokuba iingcali ziqeqeshwe;
- Imfuno yesampula ephantsi, ifuna kuphela i-50 μL;
- Iyahambelana nohlobo oluninzi lwesampulu: iSerum/Plasma/Igazi elipheleleyo.
Uvakalelo oluphezulu
- Uvakalelo: 98.95%;
- Ukuchaneka: 100%.
Isebenza kakuhle
- Ixesha lokuphendula: Imizuzu eyi-15, Ixesha lovavanyo: 10s;
- Iprotable, Usetyenziso olubanzi lweemeko;
- Ibhetri eyakhelwe-ngaphakathi, ngaphezu kweemvavanyo ezingama-200 ngaphandle kokufakwa kwamandla.
Uthembekile
- Ingqinwe ngovavanyo lwezonyango ezingama-3600, iimvavanyo ezili-1500 ngomzekelo womntu owosulelekileyo, iimvavanyo ezingama-900 kwizitofu, iimvavanyo ezili-1200 ngabantu abaqhelekileyo.;
- Idatha yezonyango ifunyenwe kubagonyeli ngesitofu esingasebenziyo, isitofu sokugonya se-nucleic acid, isitofu sokugonya seprotheyini, kunye nabantu abosulelwe yintsholongwane, abantu abaqhelekileyo.
- Ukunciphisa ixabiso lokunqanda izinga le-30%.
Umgaqo osisiseko osetyenziswa kule kit ukhuphiswano lwe-immunochromatographic.Umgca wokufumanisa (T-line) kumgca wokuvavanya ufakwe kwi-angiotensin yokuguqula i-enzyme 2 kunye nomgca wokulawula (i-C-line) ifakwe kwi-anti-rabbit ye-IgG yebhokhwe. I-conjugate pad igqunywe nge-fluorescently ebhalwe iprotein ye-COVID19 RBD kunye ne-fluorescent ebhalwe ngomvundla we-IgG antibody.Ngexesha lokufunyanwa, xa isampuli iqulethe i-antibody eza kuvavanywa, i-conjugate yento yovavanyo kwisampulu kunye ne-fluorescent antigen yenza i-immune complex kwaye i-immune complex ayinakuphinda ibophe kwi-angiotensin-converting enzyme 2 immobilized kwi-nitrocellulose membrane. .I-fluorescent antigen conjugate engabotshwanga kwi-antibody ukuba ivavanywe iya kubophelela kwi-angiotensin converting enzyme 2 immobilized kwi-nitrocellulose membrane ukwenza umgca wokufumanisa (T).
- Uvavanyo lokuhlola phambi kokugonywa;
- Ukubeka iliso kwiziphumo emva kokugonywa;
- Uvavanyo lomngcipheko wosulelo lwesibini lwabantu abosulelekileyo;
- Ukuphononongwa komngcipheko kubantu abaqhelekileyo (kubandakanywa nosulelo olungenazimpawu) nokuba nokwenzeka kosulelo;
- Uvavanyo lokukwazi ukumelana nentsholongwane.